Keywords : coli


Association Between Antibiotic Resistance and Integron Class2 Among Commonsal Escherichia coli Genotypic Groups

Al-Qadisiah Medical Journal, 2018, Volume 14, Issue 1, Pages 19-32

Antibiotic therapies can not affect only on the pathogenic bacteria, but also commensal microorganisms in the humans gut, which might serve as a reservoir of antimicrobial resistance genes. fecal Escherichia coli (E. coli) is often considered as a good indicator for selection pressure imposed by antimicrobial use. The goal of this study was to determine genotyping groups of commonsal E. coli and investigate the frequency of integrons class 2 and antibiotic resistance among them. So detect the association of integron class2 with antibiotic resistance (single and multiple drug resistance) that may be transport horizontally in bacterial populations. In this study 301isolates of commonsal E. coli were isolated from stools obtained from healthy individuals with age from 1to 80 years. All isolates were tested for their susceptibility against 16 antimicrobial agents and subjected to conventional polymerase chain reactions (PCR) for detection integrons and multiplex PCR for genotyping analysis. Present results showed that group B2 represent the majority of the collected isolates (63%) followed by group A (23%) and D (14%) but no strains were found to belong to group B1. Also results revealed that 10% of isolates have integron class 2 that mainly related to genotypic group B2. High resistance observed for most antibiotics especially ampicilln, amoxcillin, lincomycin, cephalexin while resistance to amikacin and gentamicin was less common. Antibiotic resistance mainly appeared in integron positive isolates. In conclusion, high prevalence of antibiotic resistance especially among integron class 2 positive isolates that

Antibiotics Resistance and Integron Class 1 among Commonsal Escherichia coli

Al-Qadisiah Medical Journal, 2017, Volume 13, Issue 2, Pages 30-38

Normal intestinal microbiota are a reservoir for antibiotic resistance (genes) especially Escherichia coli (E. coli). The goal of this study was to investigate the prevalence of integrons class 1 and antibiotic resistance among commensal E. coli isolates and detect the association of integron class 1 with antibiotic resistance (single or multiple drug resistance) that may be transport horizontally to other bacteria. Three hundred one E. coli isolates from stools were obtained from healthy individuals (age from 1to 80 years). All isolates were tested for their susceptibility against 16 antimicrobial agents using standard disc diffusion method and for the presence of class 1 integrons by PCR. Integrase genes detected in 112 isolates (37%) out of 301 tested isolate. High resistance observed for most antibiotics especially ampicilln, amoxcillin, lincomycin, cephalexin while resistance to amikacin and gentamicin was less common. Furthermore resistance to cefotaxime, ciprofloxacin, oxytetracyclin, tetracycline and trimethoprim significantly associated (p < 0.05) with IntI1 gene positive isolates. All integrons positive isolates are resist to more than five antibiotics and significantly associated with many resistance patterns. In conclusion, increase antibiotic resistance among commensal E. coli in this population that companied with increase frequency of integron class 1 that significantly associated with resistance for single or multiple antibiotic agents.

Antimicrobial Effect of Diode Laser and Biosynthesis Silver Nanoparticles on Escherichia Coli in Vitro

Al-Qadisiah Medical Journal, 2017, Volume 13, Issue 2, Pages 87-93

The laser and nanoparticles representing the concourse of biological science with physical science and considering modern method that used as an antibacterial instead of conventional antibiotics. The present study is an attempt to illustrate how the use of laser or silver nanoparticles each of them individually, as an antibacterial agent against Escherichia coli (E. coli) as well as study the effective result from the absorption of Diode laser energy by these nanoparticles to kill or inhibition bacterial growth. The silver nanoparticles (AgNPs) are prepared by biological method E. coli were isolated and identified by use MacConkey agar and biochemical tests. Nanoparticles are tested against E. coli cultured on Muller Hinton agar but Diode laser or laser with nanoparticles are tested against E. coli that cultured in Nutrient broth. E. coli are irradiating by Diode laser with different times. Different concentrations of silver nanoparticles have been employed for killing and inhibition bacterial growth. Results showed E. coli was effected by nanoparticles (mainly highest concentration of nanoparticles) also detected that decrease bacterial availability with increase time of exposure to Diode laser.