Keywords : Breast cancer


Utility of CA 15-3 in diagnosis of Breast Cancer Recurrence. An Observational Study

Al-Qadisiah Medical Journal, 2018, Volume 14, Issue 1, Pages 41-49

Background : CA 15-3 is the commonest tumor marker for breast cancer. Elevated serum levels of this marker was shown to be a predictor of disease recurrence in patients with localized breast cancer. This study aimed to determine the sensitivity of serum CA 15-3 level in the detection of breast cancer recurrence in relation to the molecular subtype of breast cancer, type of recurrence and the number and sites of distant metastasis.
Methods : Retrospective observational study of patients with localized breast cancer followed until relapse. Serum level of CA 15-3 at first documentation of relapse was compared between patients in respect to the molecular subtype of breast cancer, type of recurrence and the number and sites of metastasis.
Results : Elevation of CA 15-3 were found in 56% of patients at relapse. Only 6% of patients with locoregional relapse shows elevation of this marker compared with 62% of patients with distant metastasis. Elevation of CA 15-3 were more sensitive for detection of relapse in patients with luminal subtype (62%) than in patients with HER2 enriched (45%) and triple negative (35%) breast cancers. The test was more sensitive in patients with bone (69%), lung (65%) and liver (62%) metastasis than in patients with brain metastasis (25%).
Conclusions : CA 15-3 is a relatively sensitive marker for detection of breast cancer recurrence. However, this sensitivity may be hampered in patients with isolated locoregional relapses and in patients with HER2 enriched and triple negative breast cancers

Circulating microRNA-182 overexpression as a biomarker for breast cancer

Shoroq Mohammed AL-Temimi

Al-Qadisiah Medical Journal, 2015, Volume 11, Issue 2, Pages 1-10

Background:- MiR-182 is one of the most frequently studied cancer-related gene miRs and plays a crucial role in tumorigenesis , progression and may become a potential therapeutic target and biomarker of tumor diagnosis and prognosis.
Aim of study:-Estimation of miR-182 gene expression levels in both fresh tissues and serum of same breast cancer patients by using stem-loop follow by Taq-Man real time PCR (RT-PCR) technique and correlate the miR-182 gene expression with ER,PR and Her-2 by IHC technique .
Material and methods:- Stem-loop RT-PCR was performed to identify the level of miR-182 gene expression in both fresh tissues and serum of same breast cancer patients . The expression levels of miR-182 relative to mRNA of GAPDH were determined using the livak method . IHC were done for ER,PR and HER-2 .
Results:- Mean fold change of miR-182 was statistical significantly higher in breast cancer from paraneoplastic tissues , mean fold change of miR-182 was statistical significantly higher in serum of patients from apparently healthy control and miR‑182 serum level of patients with ER,PR positive was statistical significantly lower compared with the negative patients.
Conclusion:- The miR‑182 as a new original diagnostic and prognostic biomarker for breast cancer

The validity of Sonography in distinguishing benign solid breast mass from malignant

Osamah Ayad Abdulsattar

Al-Qadisiah Medical Journal, 2015, Volume 11, Issue 1, Pages 59-65

Objective:To evaluate the sensitivity and specificity, negative predictive value (NPV), and positive predictive value (PPV)of ultrasonography (US) in distinguish benign solid breast mass from malignant one.
Patients and methods: Between April 2009 and October 2012 , 243 female patients with breast lesions diagnosed by their managing surgeons, were sonographically assessed .Those who had solid lesions were selected for a prospective study through comparison with the histopathological finding of the open biopsies taken from the lesions . US features that most reliably characterize masses as benign or malignant had been strictly applied for diagnosing these cases. Sonographic classifications were compared with histopathological reports of the biopsies . The sensitivity, specificity, and negative and positive predictive values of the sonography were calculated.
Results:Sonographically ,108(44%) cases were classified as benign and 135(56%) were malignant . 12 (11%) lesions classified as benign sonographically, were found to be malignant histopathologically. 33 (24%) lesion classified as malignant were found to be benign histopathologically . Thus, the classification scheme had a negative predictive value of 89% and positive predictive value 75%.
Conclusion: Sonography could help in distinguish benign solid mass and can be follow up .

Study of BRCA1 gene expression in breast cancer in relation to some clinicopathological parameters in Al-Diwanyia city by immunohistochemistry

Maather Baqer Hussein Al-Harmooshy

Al-Qadisiah Medical Journal, 2013, Volume 9, Issue 1, Pages 13-26

Background: Breast cancer causes major part of cancer deaths in women and is increasing in incidence. The tumor suppressor gene, BRCA1 has been conferred to increase the susceptibility to breast cancer. We aimed to determine the significance of BRCA1 gene expression in relation to other prognostic factors.
Materials and methods: 45 patients with positive family history of breast cancer were selected from Al-Diwaniya Teaching Hospital / department of pathology for the study. A control group of 10 healthy subjects were also included. BRCA1 expression was assessed and correlated with age, family history , histological type and grade of breast cancer.
Results: BRCA1 was found in 9 patients 'samples (20% of the breast cancer tissues) while remaining patients (80%) were negative as well as the control group. A positive significant relationship was demonstrated between BRCA1 expression and high histological grade, age of the patient and family history and A significant negative correlation was found between BRCA1 expression and type of the tumor.
Conclusion: The study demonstrated the lack of BRCA1 gene expression in the majority of breast cancer cases and confirmed the relationship between BRCA1 expression and parameters that determine the poor prognosis in breast cancer .